Product: MAPK6/ERK3 Antibody
Catalog: AF0636
Description: Rabbit polyclonal antibody to MAPK6/ERK3
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 85kDa; 83kD(Calculated).
Uniprot: Q16659
RRID: AB_2834270

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
MAPK6/ERK3 Antibody detects endogenous levels of total MAPK6/ERK3.
RRID:
AB_2834270
Cite Format: Affinity Biosciences Cat# AF0636, RRID:AB_2834270.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ERK-3; ERK3; Extracellular signal regulated kinase 3; Extracellular signal regulated kinase p97; Extracellular signal-regulated kinase 3; HsT17250; MAP kinase 6; MAP kinase isoform p97; MAPK 6; MAPK6; Mitogen activated protein kinase 6; Mitogen-activated protein kinase 6; MK06_HUMAN; p97 MAPK; p97-MAPK; PRKM6; Protein kinase mitogen activated 5; Protein kinase mitogen activated 6;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q16659 MK06_HUMAN:

Highest expression in the skeletal muscle, followed by the brain. Also found in heart, placenta, lung, liver, pancreas, kidney and skin fibroblasts.

Description:
The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters.
Sequence:
MAEKFESLMNIHGFDLGSRYMDLKPLGCGGNGLVFSAVDNDCDKRVAIKKIVLTDPQSVKHALREIKIIRRLDHDNIVKVFEILGPSGSQLTDDVGSLTELNSVYIVQEYMETDLANVLEQGPLLEEHARLFMYQLLRGLKYIHSANVLHRDLKPANLFINTEDLVLKIGDFGLARIMDPHYSHKGHLSEGLVTKWYRSPRLLLSPNNYTKAIDMWAAGCIFAEMLTGKTLFAGAHELEQMQLILESIPVVHEEDRQELLSVIPVYIRNDMTEPHKPLTQLLPGISREALDFLEQILTFSPMDRLTAEEALSHPYMSIYSFPMDEPISSHPFHIEDEVDDILLMDETHSHIYNWERYHDCQFSEHDWPVHNNFDIDEVQLDPRALSDVTDEEEVQVDPRKYLDGDREKYLEDPAFDTNYSTEPCWQYSDHHENKYCDLECSHTCNYKTRSSSYLDNLVWRESEVNHYYEPKLIIDLSNWKEQSKEKSDKKGKSKCERNGLVKAQIALEEASQQLAGKEREKNQGFDFDSFIAGTIQLSSQHEPTDVVDKLNDLNSSVSQLELKSLISKSVSQEKQEKGMANLAQLEALYQSSWDSQFVSGGEDCFFINQFCEVRKDEQVEKENTYTSYLDKFFSRKEDTEMLETEPVEDGKLGERGHEEGFLNNSGEFLFNKQLESIGIPQFHSPVGSPLKSIQATLTPSAMKSSPQIPHQTYSSILKHLN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q16659 As Substrate

Site PTM Type Enzyme
K4 Ubiquitination
T54 Phosphorylation
K60 Ubiquitination
K141 Ubiquitination
S145 Phosphorylation
K154 Ubiquitination
Y182 Phosphorylation
S189 Phosphorylation Q16659 (MAPK6) , Q13177 (PAK2) , O75914 (PAK3)
K195 Ubiquitination
Y209 Phosphorylation
K276 Ubiquitination
Y357 Phosphorylation
S363 Phosphorylation
S386 Phosphorylation
T389 Phosphorylation
K408 Ubiquitination
K447 Ubiquitination
S450 Phosphorylation
S452 Phosphorylation
Y453 Phosphorylation
Y467 Phosphorylation
Y468 Phosphorylation
K471 Ubiquitination
K480 Ubiquitination
S483 Phosphorylation
K502 Ubiquitination
S511 Phosphorylation
K517 Ubiquitination
S556 Phosphorylation
S558 Phosphorylation
K563 Ubiquitination
K568 Ubiquitination
K621 Ubiquitination
K631 Ubiquitination
K636 Ubiquitination
T644 Phosphorylation
K651 Ubiquitination
S665 Phosphorylation
K672 Ubiquitination
S684 Phosphorylation P06493 (CDK1)
S688 Phosphorylation P06493 (CDK1)
K691 Ubiquitination
T698 Phosphorylation P06493 (CDK1)
K703 Ubiquitination
S704 Phosphorylation
S705 Phosphorylation P06493 (CDK1)

PTMs - Q16659 As Enzyme

Substrate Site Source
O95551 (TDP2) S60 Uniprot
P46695 (IER3) T18 Uniprot
Q16659 (MAPK6) S189 Uniprot
Q8IW41 (MAPKAPK5) T182 Uniprot
Q9Y6Q9 (NCOA3) S857 Uniprot

Research Backgrounds

Function:

Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK3/MAPK6 is phosphorylated at Ser-189 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6. May promote entry in the cell cycle (By similarity).

PTMs:

Phosphorylated at Ser-189 by PAK1, PAK2 and PAK3 resulting in catalytic activation. Phosphorylated by MAPKAPK5 at other sites.

Ubiquitination at Met-1 leads to degradation by the proteasome pathway.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Translocates to the cytoplasm following interaction with MAPKAPK5.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highest expression in the skeletal muscle, followed by the brain. Also found in heart, placenta, lung, liver, pancreas, kidney and skin fibroblasts.

Subunit Structure:

Heterodimer with ERK4/MAPK4. Interacts with (via FRIEDE motif) MAPKAPK5 (By similarity). Interacts with UBE3A; this interaction may be indirect and mediated by HERC2, possibly via HERC2 interaction with NEURL4.

Family&Domains:

In contrast to classical MAPKs, the TXY motif within the activation loop is replaced by the SEG motif, whose phosphorylation activates the MAP kinases.

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Research Fields

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

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