Product: ZNF174 Antibody
Catalog: AF0633
Description: Rabbit polyclonal antibody to ZNF174
Application: WB IF/ICC
Reactivity: Human, Rat
Prediction: Pig, Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 46kDa; 46kD(Calculated).
Uniprot: Q15697
RRID: AB_2834262

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Pig(91%), Bovine(80%), Sheep(80%), Rabbit(82%), Dog(90%)
Clonality:
Polyclonal
Specificity:
ZNF174 Antibody detects endogenous levels of total ZNF174.
RRID:
AB_2834262
Cite Format: Affinity Biosciences Cat# AF0633, RRID:AB_2834262.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AW-1; AW1; Zinc finger and SCAN domain containing protein 8; Zinc finger and SCAN domain-containing protein 8; Zinc finger protein 174; ZN174_HUMAN; ZNF174; ZSCAN8;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q15697 ZN174_HUMAN:

Expressed in a variety of organs, but most strongly in adult testis and ovary followed by small intestine, colon, prostate, thymus, spleen, pancreas, skeletal muscle, heart, brain and kidney. Also expressed in umbilical vein endothelial cells, foreskin fibroblast and Hep-G2 cells.

Description:
ZNF174 a transcriptional repressor of the krueppel C2H2-type zinc-finger protein family. Two splice variant isoforms have been described. Note: This description may include information from UniProtKB.
Sequence:
MAAKMEITLSSNTEASSKQERHIIAKLEEKRGPPLQKNCPDPELCRQSFRRFCYQEVSGPQEALSQLRQLCRQWLQPELHTKEQILELLVMEQFLTILPPEIQARVRHRCPMSSKEIVTLVEDFHRASKKPKQWVAVCMQGQKVLLEKTGSQLGEQELPDFQPQTPRRDLRESSPAEPSQAGAYDRLSPHHWEKSPLLQEPTPKLAGTEAPRMRSDNKENPQQEGAKGAKPCAVSAGRSKGNGLQNPEPRGANMSEPRLSRRQVSSPNAQKPFAHYQRHCRVEYISSPLKSHPLRELKKSKGGKRSLSNRLQHLGHQPTRSAKKPYKCDDCGKSFTWNSELKRHKRVHTGERPYTCGECGNCFGRQSTLKLHQRIHTGEKPYQCGQCGKSFRQSSNLHQHHRLHHGD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
91
Dog
90
Rabbit
82
Bovine
80
Sheep
80
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15697 As Substrate

Site PTM Type Enzyme
K26 Ubiquitination
T149 Phosphorylation
T165 Phosphorylation
S174 Phosphorylation
S188 Phosphorylation
S195 Phosphorylation
T202 Phosphorylation
K204 Sumoylation
S215 Phosphorylation
S266 Phosphorylation
S287 Phosphorylation
T349 Phosphorylation
S367 Phosphorylation
T368 Phosphorylation
T377 Phosphorylation
Y382 Phosphorylation

Research Backgrounds

Function:

Transcriptional repressor.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in a variety of organs, but most strongly in adult testis and ovary followed by small intestine, colon, prostate, thymus, spleen, pancreas, skeletal muscle, heart, brain and kidney. Also expressed in umbilical vein endothelial cells, foreskin fibroblast and Hep-G2 cells.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the krueppel C2H2-type zinc-finger protein family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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